Molecular and structural characterization of NADPH-dependent d-glycerate dehydrogenase from the enteric parasitic protist Entamoeba histolytica.

Novel pyrophosphate-forming acetate kinase from the protist Entamoeba histolytica.

Acetate kinase (ACK) catalyzes the reversible synthesis of acetyl phosphate by transfer of the γ-phosphate of ATP to acetate. Here we report the first biochemical and kinetic characterization of a eukaryotic ACK, that from the protist Entamoeba histolytica. Our characterization revealed that this protist ACK is the only known member of the ASKHA structural superfamily, which includes acetate ki...

Direct Molecular Detection and Phylogenetic Tree Analysis of Gastrointestinal Protozoan Parasites (Giardia lamblia, Entamoeba histolytica, Cryptosporidium parvum) from Diarrhea Infection in Kut City of Iraq: A Short Communication

Background: The intestinal tract of human can be infected by protozoan parasites. In this short communication, the stool samples were collected from patients with diarrhea referred to Kut hospital, Iraq, and then the parasites (Giardia lamblia, Entamoeba histolytica, Cryptosporidium parvum) were considered for molecular identification. Methods: Stool samples were collected from 69 patients wit...

Proteomic analysis of phagocytosis in the enteric protozoan parasite Entamoeba histolytica.

Proteomic analysis of phagosomes isolated from Entamoeba histolytica by liquid chromatography and mass spectrometry identified 85 proteins involved in surface recognition, actin cytoskeleton rearrangement, vesicular trafficking, and degradation. Phagosome localization of representative proteins was verified by immunofluorescence assay. This study should provide a basis for molecular identificat...

An Intestinal Parasitic Protist Entamoeba histolytica Possesses a Non-redundant NIF-like System for Iron-Sulfur Cluster Assembly under Anaerobic Conditions

Anti-parasitic Activity of Some Medicinal Plants Essential Oils on Giardia lamblia and Entamoeba histolytica, In Vitro

Background and objectives: Giardiasis and amoebiasis are two common diseases in human societies which make increasing problems for the health managing systems. In the present study, the antiparasitic activity of Allium sativum, Artemisia sieberi, Zatraria multiflora, Chenopodium botrys and Eucalyptus globulus essential oils on Gi...